Coding

Part:BBa_K2939001:Design

Designed by: Jacob Riina   Group: iGEM19_OhioState   (2019-10-16)


anfA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 331
    Illegal BsaI.rc site found at 610


Design Notes

EcoRI and PstI sites were changed with silent mutations to ensure biobrick compatibility


Source

Isolated for the anf genomic island of Rhodopseudomonas palustris using PCR.

References